protein extraction from muscle biopsies - protocols needed (Feb/15/2007 )

Hi everyone,

I want to extract protein from a 20 to 30mg muscle biopsie samples and I need your help to tell me how you proceed to do this experiments.

Thanks!

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protocol depends on what you are planing to do: Do you need the native filaments as well as titin to do in vitro contraction experiments including f.i. measuring myosin ATPase activity; or do you need a protocol to check protein profile where is no need of native intact proteins

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Hi

If you want extract protein you could use a homogenate method, i.e. crush your samples in Krebs-modified buffer at 4°C and centrifugate. But different methods exist by each organ... About which muscle do you speak and what the aim of your experiment?

Bye

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hi,

I need to extract protein from any skeletal muscle to make a western blot, so I don't need to have protein in native conformation.

Thanks,

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