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Is a protein of 24KD too small to serve as the antigen? - help! (Feb/12/2007 )

Hi,dear all:
I have recently purified a protein of 24KD and as the next step I want to immune rabbits to produce the antibody. The question is whether my protein is too small to serve as the antigen? I konw clone the gene into the pET-32a vector can make a fusion protein which is larger but I'm also afraid the tag may affect the speicificty of the antibody. Does anyone experienced have suggestions to me? THank you very much!

-peixumol-

QUOTE (peixumol @ Feb 13 2007, 09:39 AM)
Hi,dear all:
I have recently purified a protein of 24KD and as the next step I want to immune rabbits to produce the antibody. The question is whether my protein is too small to serve as the antigen? I konw clone the gene into the pET-32a vector can make a fusion protein which is larger but I'm also afraid the tag may affect the speicificty of the antibody. Does anyone experienced have suggestions to me? THank you very much!


Hi

I also use tagged protein antigen to select antibodies. To eliminate tag binders an unrelated tagged protein is used to verify the antigen specificity of the selected antibody.

-chick gene-

It's big enough, problems start somewhere below 5 kDa.

-K.B.-

The 24KDa protein is more than enough for producing antibodies. This also studied practically in animals. I don't know what is u r specific protein but i am working on Hep-b vaccine characterization studies where the active immunogenic antigen is a 24KDa protein and it produce antibodies.

-Sandy21-