Immortalization, SV40 - (Feb/07/2007 )

Hi,

Im in search of SV40 T antigen containing plasmid for immotalizing my cells, can anyone suggest me who would provide me with this plasmid, Im terribly in need, I cant afford the one in ATCC as my work is not funded, please help me in this regard.

Baranee

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Check the literature of people who have used this plasmid in publications, and email the corresponding authors. Usually people are cooperative if they have things.

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Hi, Thanxs for the reply.
I Have tried corresponding atleast six of the authors ! unfortunately none have replied me back!!! . Thats why I posted this message here. I did get 2 other plasmids from the authors, Only for this SV40 Im not able to get! I dont know what to do next. Getting down from ATCC will be too expensive for me and Im not in a position to pocket it out! Suggest me what to do.

Thanxs

Baranee

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that is unusual to get no positive feedback from so many authors; authors hesitate if someone is new in the field, or a stuff is also commercial available, or you missed to work out why you especially asked this author;

may be your supervisor can help to ask authors, or you find out if someone else in your institute/clinic is working with SV40, in most cases it will go fast

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Thanxs for the message,
Well you are true, Im quite new in this field and also SV40 is commercially available. The only thing was its too expensive for me I couldnt afford. Thats why I kept mailing. Other thing as you stated i would have missed to work out as why I especially asked the author. But now I cant give them second mail, it wouldnt be nice!. Nobody else here in my institute or near by are working with this.
And if I told this to my supervisor he would ask me to forget the work and carry on to next. But this particular work would be a novel and substantial part in my PhD thats why I couldnt give up. Please try to help in this regard.

Thanx in advance,

Baranee

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the only thing in your case - if you have no money to buy - is to ask other authors, there are many who work with SV40, and explain why you need this vector and explain your situation; do it in an official letter from your institute, mail it by pdf or as regular postage, not as a simple email; do not ask the same authors again

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Maybe try Addgene: their plasmids are often less $$$$

http://www.addgene.org

mito 1

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Thanx everyone! atleast one of the author seem to have responded to my mail.

Regards

Baranee

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