Visualizing the cytoskeleton - (Feb/01/2007 )
Hi,
I have transfected some neurons with some GFP-bound proteins such as p150glued, clip170, EB3 and EB1 and so far it looks as if they are binding all the way along microtubules. I am quite surprised cause I thought those were proteins binding to the extremities of microtubules. Does anyone here have any experience with those proteins?
-Edwige-
QUOTE (Edwige @ Feb 1 2007, 07:18 PM)
Hi,
I have transfected some neurons with some GFP-bound proteins such as p150glued, clip170, EB3 and EB1 and so far it looks as if they are binding all the way along microtubules. I am quite surprised cause I thought those were proteins binding to the extremities of microtubules. Does anyone here have any experience with those proteins?
I have transfected some neurons with some GFP-bound proteins such as p150glued, clip170, EB3 and EB1 and so far it looks as if they are binding all the way along microtubules. I am quite surprised cause I thought those were proteins binding to the extremities of microtubules. Does anyone here have any experience with those proteins?
you mean transfection of expression vectors for GFP-fused proteins? may GFP bind to microtubules? how was the distribution of GFP-fused proteins after destruction of microtubules?
-The Bearer-
QUOTE (The Bearer @ Feb 2 2007, 05:26 AM)
QUOTE (Edwige @ Feb 1 2007, 07:18 PM)
Hi,
I have transfected some neurons with some GFP-bound proteins such as p150glued, clip170, EB3 and EB1 and so far it looks as if they are binding all the way along microtubules. I am quite surprised cause I thought those were proteins binding to the extremities of microtubules. Does anyone here have any experience with those proteins?
I have transfected some neurons with some GFP-bound proteins such as p150glued, clip170, EB3 and EB1 and so far it looks as if they are binding all the way along microtubules. I am quite surprised cause I thought those were proteins binding to the extremities of microtubules. Does anyone here have any experience with those proteins?
you mean transfection of expression vectors for GFP-fused proteins? may GFP bind to microtubules? how was the distribution of GFP-fused proteins after destruction of microtubules?
I mean that I transfected with GFP-p150glued, GFP-clip170, etc... I wonder if that is the normal distribution pattern of those proteins on the neuronal cytoskeleton.
-Edwige-