A431 Transfection - any good protocol known for A431 (Jan/17/2007 )

Hey,

since a couple of days I'm trying to transfect A431 cells with some expression plasmids.
However, to do a good transfection a.s.o I first transfect GFP as a efficiancy control. But actually it s#cks. There are billions of recommendations what I could do, but I thought maybe some of you might have a good protocol with a sky-high transfection rate for these guys...I would like to avoid nucleofection.

Thanks for any advice and support
Cheers

With some optimization, a 20-30% positive rate is achievable with Lipo2000. Not much higher than that though.