do you ever use PAGE to check PCR? - (Dec/20/2006 )
one guy in our lab advices all new students who come to use PAGE to simply check the PCR reaction. 
i always use agarose, it is cheap, quick and not health hazard as acrylamide. dont you think it is a waste of money and health?
-Kathy-
Definitely,
But the PCR based assay which I use needs polyacrylamide gel as the expected result is a ladder f bands ranging from 36bp and above with 6bp difference. Otherwise if amplicon is above 100bp which is the usual case agarose gel is used.
-Calvin*-
QUOTE (Calvin* @ Dec 20 2006, 09:33 PM)
Definitely,
But the PCR based assay which I use needs polyacrylamide gel as the expected result is a ladder f bands ranging from 36bp and above with 6bp difference. Otherwise if amplicon is above 100bp which is the usual case agarose gel is used.
But the PCR based assay which I use needs polyacrylamide gel as the expected result is a ladder f bands ranging from 36bp and above with 6bp difference. Otherwise if amplicon is above 100bp which is the usual case agarose gel is used.
actually yes, very simple 300bp-1Kbp PCR products and all new students are bothered by preparing acrylamide. i think he likes to complicate his and everyones life so much
-Kathy-
PAGE is recommanded for small size fragments, such as around 100bps
while for larger size fragment, using PAGE is not wise idea
-rye-