My publication - About my published paper/poster (open discussion) (Dec/20/2006 )
The Effect of Seahorse (Hippocampus kuda Bleeker 1852) Extract
On Spermatogenesis of Mice (Mus musculus L.)
Laksmindra Fitria
Laboratory of Animal Physiology, Faculty of Biology, Gadjah Mada University
Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281 Indonesia
Phone/Fax: 62-274-580839, E-mail: laksmindra.fitria@yahoo.com
ABSTRACT
For centuries, it has been believed that seahorse can be used to deal with male sexual dysfunction. However, there is still less scientific evidence to provide this myth. This research was carried out to study the effect of seahorse extract on spermatogenesis of mice. Balanced-factorial-CRD was applied for the experiment design. Sixty three-month-old male mice were divided into two groups. Each group was divided into six subgroups. Four subgroups were for treatment, the rest two subgroups were for controls. Seahorse was extracted using maceration method and was dissolved into distilled water to get final dose 100; 150; 200; 250 mg/kg BW. Extract was administered orally 0.5 mL/day for 34 and 67 days. Control groups were supplied with tap water or distilled water (placebos). On day 35 and 68, mice were sacrificed. Testes were taken and were processed for histological preparation in paraffin method using fixative Bouin and HE staining. Number of spermatogonia, spermatocytes, spermatids, and total spermatogenic cells were counted. Data were analyzed statistically using ANOVA and continued with LSD. Result showed that number of spermatogenic cells from all treatment groups significantly increased with increasing dose and duration of treatment (linear). In control groups, number of spermatogenic cells significantly decreased with increasing duration of treatment. Optimum treatment was obtained on dose 200 mg/kg BW, which was administered for 67 days. It was concluded that seahorse extract positively affected on increasing and maintaining spermatogenesis on mice. By consuming seahorse regularly as a daily menu could improve potential of fertility on male individual.
Keywords: seahorse, extract, mice, spermatogenesis
__________________________________________________
Presented to International Seminar and Workshop: “Marine Biodiversity and the Potential For Developing Bio-Pharmaceutical Industry in Indonesia" on May 17-18 2006, Kompleks Bidakara, Jakarta.
Poor seahorses. 
I like them.
Hopefully they are from a rearing??
On Spermatogenesis of Mice (Mus musculus L.)
Laksmindra Fitria
Laboratory of Animal Physiology, Faculty of Biology, Gadjah Mada University
Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281 Indonesia
Phone/Fax: 62-274-580839, E-mail: laksmindra.fitria@yahoo.com
ABSTRACT
For centuries, it has been believed that seahorse can be used to deal with male sexual dysfunction. However, there is still less scientific evidence to provide this myth. This research was carried out to study the effect of seahorse extract on spermatogenesis of mice. Balanced-factorial-CRD was applied for the experiment design. Sixty three-month-old male mice were divided into two groups. Each group was divided into six subgroups. Four subgroups were for treatment, the rest two subgroups were for controls. Seahorse was extracted using maceration method and was dissolved into distilled water to get final dose 100; 150; 200; 250 mg/kg BW. Extract was administered orally 0.5 mL/day for 34 and 67 days. Control groups were supplied with tap water or distilled water (placebos). On day 35 and 68, mice were sacrificed. Testes were taken and were processed for histological preparation in paraffin method using fixative Bouin and HE staining. Number of spermatogonia, spermatocytes, spermatids, and total spermatogenic cells were counted. Data were analyzed statistically using ANOVA and continued with LSD. Result showed that number of spermatogenic cells from all treatment groups significantly increased with increasing dose and duration of treatment (linear). In control groups, number of spermatogenic cells significantly decreased with increasing duration of treatment. Optimum treatment was obtained on dose 200 mg/kg BW, which was administered for 67 days. It was concluded that seahorse extract positively affected on increasing and maintaining spermatogenesis on mice. By consuming seahorse regularly as a daily menu could improve potential of fertility on male individual.
Keywords: seahorse, extract, mice, spermatogenesis
__________________________________________________
Presented to International Seminar and Workshop: “Marine Biodiversity and the Potential For Developing Bio-Pharmaceutical Industry in Indonesia" on May 17-18 2006, Kompleks Bidakara, Jakarta.
Maceration of seahorses?? 
Why the whole seahorse instead of their gonads or hormones?
I like them.Hopefully they are from a rearing??
Don't worry, fella. I am into Conservation Biology as well. I never harvest my seahorses from the wild. I know seahorse is a vulnerable species. However, it is reported that this special fish is very potential for improving health. Thus, we develop aquaculture for seahorse farming. They grow fast and pretty well here. We also study how to take care of them as good as they should get by feeding with good stuff and watch their environment. In the other hand, this seafarming recruits many local people to get job, so they like to help us. A mutual cooperation
Why the whole seahorse instead of their gonads or hormones?
The research was based on how people consume seahorses as daily menu. They use the whole body as food or beverage. I made it as extract or "concentrate" so I can watch the effect clearly and no need to apply a lot of seahorses during the research. Also, sin she (Chinese medical doctor) likes to grind seahorses into powder, pack them in pills or capsules for patients who cannot consume it directly as fish menu.