Anyone ever do a molecular beacon PCR? - (Dec/15/2006 )
if so what kind of reaction/cycling conditions do you use?
thanks
-BioDude1-
What machine are you using?
If you are using a fast-cycler like the Roche lightcycler, your extension times can be 0 to 5 seconds, with a 1 degree per second melt step at the end, even slower if you want.
-swanny-