Protocol Online logo
Top : Forum Archives: : Cell Biology

i want to c the proliferation of concanavalin A on the HepG2 - (Dec/05/2006 )

hi all


i want to see the proliferaton of HepG2 by concanavalin A. but i m not getting the relevant literature.

cud someone help me in this regard.



thanx in avance.

-pBS-

I believe most works on cell proliferation stimulatory effect by conA or PHA are done using lymphocytes.

-genehunter-1-

yes you are right.


but in my experiment i have to check the proliferation of my cells in response to one antiproliferative agents and one proliferative agents.


if u suggest some other proliferative agent then its very kind.


and i want to know that which cocn of the concanavalin should be used for lymphocytes.

-pBS-

HGF or EGF would be better stimuli for HepG2 cells. TPA would do too, but it may be dose dependent.

-genehunter-1-