CBB DYE BOUND TO PROTEIN - (Dec/01/2006 )

In staining the gel using coomassie brilliant blue R 250,How protein are stained and what ia mechanism of dye -protein complex.

Muthuraman

When CBB binds to proteins at low pH values, a reddish protein-binding species is thought to be converted to a blue protein-bound species. An interconversion between two differently-colored ionization states of the dye molecule is the simplest explanation of the absorbance shift which occurs during binding, however the exact mechanism of action is still not understood according to my knowldge.
hope this help.

Check the following link.

http://nationaldiagnostics.com/article_inf.../articles_id/80