RNase and DNase free? - water (Nov/29/2006 )
Dear all,
I have an Elga UltraPure Genetic water purification system in my lab. According to the equipment handbook, the water from the system is supposed to be DNase and RNase free. (I don't have the means to verify this). I was wondering how could the purification system able to eliminates RNase and DNase. I am about to start my RNA works. I planned to DEPC treat and double autoclave the water as a precaution. Do you think it's necessary? I appreciate all the comments. Thank you.
PS: The water works well for the proteomics.
-idlewild-
QUOTE (idlewild @ Nov 29 2006, 05:57 PM)
Dear all,
I have an Elga UltraPure Genetic water purification system in my lab. According to the equipment handbook, the water from the system is supposed to be DNase and RNase free. (I don't have the means to verify this). I was wondering how could the purification system able to eliminates RNase and DNase. I am about to start my RNA works. I planned to DEPC treat and double autoclave the water as a precaution. Do you think it's necessary? I appreciate all the comments. Thank you.
PS: The water works well for the proteomics.
I have an Elga UltraPure Genetic water purification system in my lab. According to the equipment handbook, the water from the system is supposed to be DNase and RNase free. (I don't have the means to verify this). I was wondering how could the purification system able to eliminates RNase and DNase. I am about to start my RNA works. I planned to DEPC treat and double autoclave the water as a precaution. Do you think it's necessary? I appreciate all the comments. Thank you.
PS: The water works well for the proteomics.
Yes. It is good to be sure the water is RNAse and DNAse free by treating it with DEPC.
-Minnie Mouse-
i never satisfied with one time autoclave of DEPC treated water. i always done 2 or 3 times autoclave.
-T. reesei-