About two-step Chip - (Nov/29/2006 )
I want to use one antibody angait TFs to immunoprecipitate chromatin and then use another TF antibody to chip again.
In one paper, they use TE with DTT to dissociate protein A -antibody -chromatin complexes and get free chromatin.
Any suggestion to release chromatin from protein A-antibody and do two-step chip?
Thanks a alot!
-hysteria-
QUOTE (hysteria @ Nov 29 2006, 07:35 AM)
I want to use one antibody angait TFs to immunoprecipitate chromatin and then use another TF antibody to chip again.
In one paper, they use TE with DTT to dissociate protein A -antibody -chromatin complexes and get free chromatin.
Any suggestion to release chromatin from protein A-antibody and do two-step chip?
Thanks a alot!
In one paper, they use TE with DTT to dissociate protein A -antibody -chromatin complexes and get free chromatin.
Any suggestion to release chromatin from protein A-antibody and do two-step chip?
Thanks a alot!
Have you seen this publication.
NAR 32(19) e151
Quantitative sequential chromatin immunoprecipitation, a method for analyzing co-occupancy of proteins at genomic regions in vivo
Joseph V. Geisberg and Kevin Struhl
-KPDE-