restriction map - (Nov/27/2006 )

hi....

i have some qestions (not H.W) and i want to be sure about my solutions..if possible
for this sequence...

1) BamHI sites: 2sites: 711,1243
2)EcoRI sites: 2sites:1628,326
3)how many orf: 5
4)which orf is the correct one: can't understand this one ?

that'a all ...thanx in advance

hi
i encounter difficulties because it seems the file is corrupted.
May you either reattach it (full edit function of the board) or pm it to me?
thanks.

ok let's sum up
1920bp sequence

BamHI (G'GATC_C)
Cuts 1 time.
Cuts at position 693.
Fragment sizes 693, 1227

EcoRI (G'AATT_C) [FunII]
Cuts 1 time.
Cuts at position 1610.
Fragment sizes 1610, 310.

so it seems you didn't send me the same?...
i think the ORF sequence matches the first ATG encounteredas checked by pubmed...

no, it's the same sequence i used...how did u get these numbers fred?
i tried bioinformatics.vg site..

well i paste the sequence in pDRAW (available at acaclone) and results came directly.
For checking the ORF, i did a blast search.

ok...thanx fred..i check your program also, but i don't know why mines are different...anyway, your results seem right...but i want on what base we choose this orf or the other one as the "correct one"?!

here the are in txt word and OPEN DOC format