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testing CHIP samples on Western - how to de-crosslink (Nov/21/2006 )

Hello,

I would like to test my CHIP samples after crosslinking and IP on Western. Is it sufficient to de-crosslink by boiling in sample buffer? Or do I need to do something else?

Thanks for your advice,

Theo

-Theo22-

QUOTE (Theo22 @ Nov 21 2006, 09:56 PM)
Hello,

I would like to test my CHIP samples after crosslinking and IP on Western. Is it sufficient to de-crosslink by boiling in sample buffer? Or do I need to do something else?

Thanks for your advice,

Theo


We have discussed the topic a few months ago. You should check previous posts...

-dnafactory-

to help you along

here is the link on one of the discussions Theo was referring to

Western Discussion

-methylnick-