testing CHIP samples on Western - how to de-crosslink (Nov/21/2006 )
Hello,
I would like to test my CHIP samples after crosslinking and IP on Western. Is it sufficient to de-crosslink by boiling in sample buffer? Or do I need to do something else?
Thanks for your advice,
Theo
-Theo22-
QUOTE (Theo22 @ Nov 21 2006, 09:56 PM)
Hello,
I would like to test my CHIP samples after crosslinking and IP on Western. Is it sufficient to de-crosslink by boiling in sample buffer? Or do I need to do something else?
Thanks for your advice,
Theo
I would like to test my CHIP samples after crosslinking and IP on Western. Is it sufficient to de-crosslink by boiling in sample buffer? Or do I need to do something else?
Thanks for your advice,
Theo
We have discussed the topic a few months ago. You should check previous posts...
-dnafactory-
to help you along
here is the link on one of the discussions Theo was referring to
Western Discussion
-methylnick-