standalone blast help needed - cant open .nin (Nov/20/2006 )

Dear folks,

I am trying to run a standalone blast on my Mac laptop.
I have downloaded the executables and ecoli.nt files from ncbi.
I created a blast.ncbirc file which is in my root directory and has the following content:
[NCBI]
Data=/Users/teresa/blast/blast2.2.15/data


The ecoli data has been formatted and has created the 7 expected files (.nin, .nsq, etc. These files appear to be ok, as far as I can tell. The .nsq is ~ 1mb, the others are 4-60kb).

When i try to run blastall this is what happens..

dhcp48:~ teresa$ blast/blast2.2.15/bin/blastall -p blastn -d blast/ecoli.nt -i blast/bacs/001D22.rtf
[NULL_Caption] WARNING: Unable to open ecoli.nt.nin

I have formatting a different dataset and using that as well, but i get the same error.

apologies for what is probably a reflection of my limited unix skills, but any advice would be much appreciated!!
teresa

---

the path to your database is not correct.

---

go to where your database is and type:
scruffy@joshua~ pwd

= /home/scruffy/testdata/ # you should get something like that.
replace your -d with

/home/scruffy/testdata/database.flatfile.name
where database.flatfile.name is the name of the input file you passed to formatdb

---

thanks! working now....

---