ss DNA cutting endonucleases - Mbo I? (Nov/09/2006 )
Hi
Does somebody know where I can check whether the restriction endonuclease I use can digest ssDNA?
I haven't found anything about this kind of activity for Mbo I, does somebody know if it has it?
Thanks
-tedijp-
Restriction enzymes, in general, will not cut ssDNA unless the DNA forms hairpins or dimers which are locally dsDNA having the RE recognition site.
-phage434-
MboI does not cut ssDNA.... unless the ssDNA foldsback on itself or dimerises to form small local dsDNA regions
-perneseblue-