humanise - DNA (Nov/06/2006 )

I heard someone telling I have to humanise my sequence.
what does it mean? any exemple?

thanks


jm

anyone?

Maybe it has to do with codon usage? What organism was the DNA originally from and what are you going to do with it?

I don't have any organism or what ever.
I just heard and Iam curious about it and wanted to know more.

humanise a DNA sequence it maybe a to modified the DNA in order to get the most used codon for an aa in human. its' a guess.
let say you have a sequence from mouse where the codon use for D is GAT. the human codon use more frequently GAC.
So if I change every D codon in GAC , am I humanising my sequence?

if someone could give me an info about this will be nice

thanks

Humanise a sequence...

that in my mind usually means the promoter, terminator and poly A signal. Change those sequences from that belonging to what ever mammalian species the gene was taken from to that which is human for good expression in human cells.

I don't think codon usage is such a big matter in human/mammalian systems... really there hasn't been that much time since the mammalian radiation 65 million years ago.

Humanise my protein... (in relation to antibodies)
well that means changing the appropriate domains of the protein to the human version.

I agree codon usage within the human/mammalian species hasn't changed too much, but with genes from for instance bacteria you want for some reason to express in a mammalian cell, you might want to alter codon usage here and there...