rat's umbilical cord culture - (Nov/05/2006 )

hi.
I want to start to culture rats umbilical cord, but I don't know how to do it. is there anybody experienced in culturing the umbilical cord? can share information with me...
thanks.

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aha, I have cultured umbilical cord mesenchymal cells for a long time. If you have some questions, I will be glad to discuss them with you.

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Are you going to culture rat umbilical cord endothelai cells? If yes, you can use collagenase.

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From what species did you culture these cells? Did you also culture mesenchymal stem cells from bone?

Until now I only isolated MSCs from bone. Next week I may get umbilical cords for collecting cells. The method seems to be the same, but maybe you know some important things I have to keep in mind.

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Hi~

I'm now using the human MSC-derived from umbilical cord blood, and I want to add the in vivo experiment.

Could you tell me the method culturing MSC in rat or mouse??

-Jungs

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