Protocol Online logo
Top : Forum Archives: : Molecular Biology

mRNA quantification - mRNA quantification (Oct/14/2002 )

Dear all,

Who knows what is the best technique to determine mRNA concentration?

Thanks in advance

Peter

-peterj-

Hey

Easiest way is to use a spectrophotometer. Take readings at both 260nm and 280 nm. Protocol is very common. Simple calculatoin to determine quantity of RNA, as well as purity!

Tom

-welchtom-