Melting Curve/Sample analysis Question (PCR) - (Oct/30/2006 )

Hi-

If i wanted to end a pcr run with a melting curve analysis, would that affect my ability to visualize my samples on an agarose gel? i.e...would the melting curve alter or change the physical properties of a PCR products?

thanks

-BioDude1-

QUOTE (BioDude1 @ Oct 30 2006, 07:08 PM)

The melting curve is very short and it consists of 3 steps (95-60-95) very fast. I always have it at the end of my realtime. It will not affect the properties of the products.

-dnafactory-

Since the sequences are simply melting then rehybridizing, it should be the same sequence at the end of the run

-tap14-

great! thanks

-BioDude1-