Genomic DNA Degradation - (Oct/29/2006 )

Hi, I have been trying to PCR a region from some mouse genomic DNA that I was given but have had no luck. So I ran a sample of the genomic DNA on a gel. I found a bright band high up on the gel followed by a large smear. Is this genomic DNA any good to be used as a template for PCR? Thanks!

Hi,

Could be the genomic, and could be the PCR too. If you have positive controls, that'd be nice.

I think, sometimes, it's possible to get PCR product from a degraded gDNA (partial degradation). If the region is GC rich, try optimising with additives etc. Also, make sure initial denaturing step is long (10 to 15 min or longer?)

Thanks 'I love MSGs'... I'll give your suggestions a try.