7AAD and PI - (Oct/24/2006 )
Dear all
I'm performing FACs analysis for cell cycle study. For determining DNA content i can use PI or 7AAD. But what is the difference between PI and 7AAD? Have i to treat samples whit DNasi or RNasi before performing facs analysis?
Thank in advance! all the best.
-nonsensey-
you cannot block all polymerases by actinomycine D.
At least shoul you use alpha amanitine too.
-fred_33-