look for protocol of glycero-gradient ultracentrifugation - (Oct/10/2006 )

Hi, everyone:
I want to use glycerol-gradient ultracentrifugation for fractionlation my cell lysate. but I don't know how to do it. Would anyone provide me some protocol or any experience?


Thank you very much!

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It is easy to do. It is just time consuming.

Lysis buffer may be different upon different protein.

Just a little tip for you: U may make 50% glycerol by mixing which with your lysis buffer.Otherwise it will be very hard to get right volume of glycerol. U may make master mix for different layer and then add to your centifuge tube layer by layer slowly.

Good luck!

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