Protein refolding - (Oct/08/2006 )

Can any body tell me some easy to use protocols for protein folding

---

Can you give us some background please, so we can work out what the issues are?

---

I am trying to express a new protein. But its soluble expressionis very low and protein goes into inclusion body evern at cold room temperature. Protein in inclusionbody is in large amount. So first I want to get protein from inclusion body and try to refold it. or i will express chaperones with my genes to increase it soluble expression.
with best regards
from
samita

---