Protocol for irradiated APC from spleen - (Sep/27/2006 )

Hi
I was wondering how to prepare irradited APC, Iwant to use these for costimulation to differntiate cells to Th1 lineage. Also how does CD90 depletion affect costimulation

Thanks In advance
Akanksha

I don't know if it's a general procedure, we used 3000 rad to irradiate APC for lymphocyte stimulation.