CTAB - function of CTAB in DNA extraction (Sep/27/2006 )
Hi,
I try to figure out what is the function of CTAB in DNA extraction?
Why we only use it mostly in plant extraction?
is there any other uses of CTAB?
Thanks
-oche-
CTAB is a detergent that helps lyse the cell membrane, however it is pretty poor with denaturing proteins so something with a longer tail is usually used for extraction.
-mandolin-
QUOTE (mandolin @ Sep 28 2006, 06:24 AM)
CTAB is a detergent that helps lyse the cell membrane, however it is pretty poor with denaturing proteins so something with a longer tail is usually used for extraction.
According to my opinion CTAB-NaCl solution binds with the Proteins in the digested Cell lysate and helps in seperation of DNA from Protein making intermediate ring of protein. Hope this helps u

-sanjiv kumar-