SNP scanning using HRMA - (Sep/25/2006 )

Hi, I am very interested in the High Resolution Melting Analysis for SNP scanning and genotyping.
HRM characterizes nucleic acid samples based on their disassociation (melting) behavior. It is a PCR-based technology and saturating dyes such as SYTO® 9 EvaGreen™ and LC Green® are used at higher concentrations to provide increased resolution over traditional dyes like SYBR® Green 1.

There are few questions here:

1) Is this a new technology in SNP scanning? Is anyone heard before this HRMA?

2) Is anyone from UK or Malaysia working on this HRMA (using RotorGene™ 6000 (Corbett Research), HR-1™ and 384 well LightScanner™ (both from Idaho Technology)??)

Really appreciate if anyone can share your information/expericence of HRMA with me.

Thanks.

Rachel

I am also in need of this mean.

analysing SNPs by their melting point is not new, just ask any pathologist.
High-Resolution Melting Analysis, using the specific dyes you mentioned, is new... the earliest paper i found was published in 2005.

V

Hi.
I am interested too.
The machines (Idaho Techn.) are new. But about the method, I heard it exists for a long time.
If somebody can say something more about experience with it, i will be very interested to read it.