RNA at 50 degree overnite, degraded? - (Sep/17/2006 )

i have extracted RNA from cell line and accidentally left it overnight at 50 degree water bath.

do u think the RNA still can be used ?

Argh.. i very reluctant to run a RNA GEL to check.. Any idea?

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Your RNA is probably degraded.

You may run this RNA sample if you have a spare well in a RNA gel.

Hope this help.

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Agreed.

As Dr McCoy would say, "He's dead Jim"

But as Minnie suggested, run it in a spare lane.

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You need to run it on a gel to know whether it is degraded or not (probably it is). No alternatives.

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