Longer time and higher temperature for flow cytometry. - what will happen (Sep/12/2006 )

For flowcytometry, we ususally incubate cells and the antibodies at 4 degree C for 30 min to 1 hr.

What will happen if we do it for longer time, let us say 48 hrs and what will happen if we keep them at 37 degrees C?

What is the most reasonable time for this?

Thank You!

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If doing cell surface staining, Ab may internalize into the cell at 37 degree.

Hope this may help.

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Thank U

That was helpful and I guess even the survival of the cell may be compromised at higher temperature . . . and the rule of thumb is Cold for Ab and Hot for Enz, if I am right . .

I wonder what will happen at longer time.

And what is the optimal time.

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