Which direction ethedium bromide moves? - (Sep/03/2006 )

[size=2]Hi...
If Ethedium Bromide is mixed with agarose gel during electrophoresis in which direction it moves?
is it anode towards cathode or visa versa?
Which staining is better? staining after electrophoresis or during electrophoresis?

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The EtBr moves toward the negative pole

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Staining before electrophoresis is the normal protocol followed as observed by me.
Try staining after and tel me the result.

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EtBr moves toward the negative pole. I prefere the staining before electrophoresis better than the one afterwards

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Thank u.... I got it , I will definitly tell u the result of after staining

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Thank u ... Igot it.... I got the doubt of staining procedure because most of protocal books describe after staining.

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i prepare the running buffer with EtBr, and dissolve agarose with this same buffer; it is working fine
staining after electrophoresis is possible, but it the bath takes some time, the material diffuses outside the gel and the bands are not as bright as with the first method
Seb

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Ya.... I got it thanks

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If you are looking for one band only, or bands of similar sizes (less than log diff) EtBr in the loading dye gives the best results.
Little or no background! Just remember to have some in your ladder too.

Since the binding of EtBr is a dynamic happening, and ETBr moves in oposite direction of DNA, large DNA molecules will catch more EtBr than small molecules with this method (more so than with any of the "staining the whole gel"-methods.

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