what are dye blobs? - (Aug/26/2006 )

HI!
What are dye blobs? Someone told me that it is a second labled-ddNTP, binding to a labled sequence,...
I was not able to find something in the manual of the sequencer, and nothing in the book with course material,...

Thank's for your help

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does this answers your question?
http://www.nucleics.com/DNA_sequencing_sup...-dye-blobs.html

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HI!
Thats exactly the page i read, before i started this topic. There is no real answer what dye blobs are. In The AB-Users Manual, there was also only a picture of raw data (there you can see why it is called "blob" ;-) ), but i do not understand the chemistry behind it,...
Are dye-blobs more than one dye-molecule? (I think so, because dyeblob appear in different stages of the sequencing) - but how do they bind together? Or are the bound to DNA-strands?

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