Nuclease free water for PCR - DEPC treated vs non DEPC treated (Aug/12/2006 )

hi guys!!

i juz want to know the difference btw these type of nuclease free water. which one give better results in PCR? and for sequencing also.. thanks

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U use DEPC if you are dealing with RNA, I never heard of doing PCR with DEPC water, in some cases can even inhibit enzymatic activity

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Normal a sterile water is used for PCR and even for preparation sequencing reactions One uses nuclease free when working with RNA, from cDNA onwards use the normal sterile water.

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We use DEPC water for all things with RNA and everything else autoclaved or Millipore water.

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DEPC is degraded by autoclave in alcohol substance and other stuff. Thus this alcohol may inhibit partially or fully the PCR reaction.

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I use DEPC water for PCR and it words well

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We use bidistilled water for PCR, no problems. It is not autoclaved and but frozen in aliquots. You can also buy nuclease free water from companies.

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