Protocol:GFP reporter assay - (Aug/10/2006 )

Hi,

Can anyone provide me with the detail protocol of GFP reporter assay.

Thanks

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Simple, examine your cells under a fluorescence microscope using FITC (green) filter.

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Thanks, one more stupid question. Can I do it in a 96- well plate and What is the wavelength?

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yes,

ur microscope will have a filter for it.

excitation peak at 395 nm & emission peak is at 509 nm-GFP

excitation peak is 490 nm with the peak emission kept at 509 nm - EGFP

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our scope has 480/535 set of filters.

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