a blue dye for confocal - (Aug/07/2006 )
dear friends,
I want conjugate a blue dye to a lipid which used as a marker in confocal. Which dye (blue) should I use? Is pacific blue OK? thank you very much.
-zky-
QUOTE (zky @ Aug 8 2006, 05:16 AM)
dear friends,
I want conjugate a blue dye to a lipid which used as a marker in confocal. Which dye (blue) should I use? Is pacific blue OK? thank you very much.
I want conjugate a blue dye to a lipid which used as a marker in confocal. Which dye (blue) should I use? Is pacific blue OK? thank you very much.
Do you mean a dye which is actually blue or that absorbs and emits at the blue end of the visible spectrum?
-Doc_Martin-
It depends on the laser you have, and the other labeling you want to do.
have a look here, to see the spectra of some dye I like.
http://probes.invitrogen.com/resources/spectraviewer/
-Missele-