Microarray data Analysis - Data's for life (Jul/27/2006 )

Hi everyone,

I really want to know something abt. the data analysing part in micro array experiments...... and the statistical techniques involved...


arul.

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hi arul,

I think its a vast area ur asking for... I would suggest you to read few research articles that speaks abt data analysis, and try to interpret a single research artilce with a free ware available (like BRB). I think experts here can help u if ur struck somewhr in the middle.

Regards,
Ganesh

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Hello Arul,

Check out this really cool site which has a software for Microarrray data analysis.

http://www.ocimumbio.com/web/bioinformatic...&prodType=1


Hope this is helpful to you.

Oligo

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hello
I read articles about microarray. I wonder what 22K or 44K microarray means and what "spke-in" means?
please

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22K means that microarray contains ~22000 gene probes. Spike-in normally refers to external cRNA or cDNA or RNA used as quality control for amplification or hybridisation or IVT.

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The “R” program as well as "Bioconductor" will be useful in data preprocessing, differential expression, and identifying differentially expressed genes using Affymetrix and 2-channel arrays. The other one is Limma. The multivariate analysis can be performed as well. Programs are available on the Biocoductor web site.

These programs require some computational knowledge, but is easy to learn the basic R code and write own comands. Good luck!

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