reporter assay - (Jun/25/2002 )

I have cloned promotor sequences in the pGL3 basic plasmid. In one case, I have less luciferase activity with my promotor construct than with the pGL3 basic plasmid, even after normalisation with a cotransfected pCMV beta gal plasmid. Could this data be relevant?

(Edited by muriel at 7:11 am on June 25, 2001)

G'Day Muriel! Sorry I cannot be of help considering your question. But could you please give me the protocol and a little background on the normalisation with ß-gal? Feel free to send me an email: boettcher@imt.uni-marburg.de
Kindest regards, Ingo