PEG to seperate Plasmide from Protein? - (Jul/14/2006 )
I was told you can use PEG tp precipitate protein when you have a plasmide purification that contains a bigger protein contamination (precipitating the DNA yields a pellet almost impossible to resolve and there is a bad 260/280nm ratio for the solution).
Is that true?
I only found informations of how to precipitate DNA from plasmide preps with PEG8000 and NaCl. But I would like to precipitate the protein inside and than just do a Isoprop precipitation of the remaining plasmide.
Anyone has a protocol or hint how to do that if its possible at all?
-wincel-
a cleaner method is to use phenol chloroform iaa...
isn't it feasible?
-fred_33-
QUOTE (fred_33 @ Jul 19 2006, 07:07 PM)
a cleaner method is to use phenol chloroform iaa...
isn't it feasible?
isn't it feasible?
It would be, if we did not have to prevent precipitation as the pellet isn't solving anymore and we have a maxi prep with 10,5ml of Eluate - this would need tons of Chlo/Phe/etc. I hoped PEG is possible with smaller Volumes?
-wincel-