questions about L-Cells - (Jul/13/2006 )

Hi,
I am culturing L-cells, which seems to have funny phenomena....

one dish, I left in the 37 incubator for one week by mistake, I found the medium became viscous, while the cells were living happly.... I mean, they made the medium viscous.... But I have no idea, what that can be... secrated substance?

another dish, by another mistake, I put the cells in a non-coated petri dish, they seem to become suspension cells, also living happyly and dividiing and proliferating....

Does anyone have similar experience with this or other cell lines?

Cheers,
Yeping

Interesting observations. Are these close to L929 cells? I had similar experience with L929 when it was left unattended for a long period of time. Sometimes a second batch of cells start to grow after initial growth are gone. Visicous seems to come from released DNA originated from the dead cells.

Have you measure the growth curve for the cells in suspension, or it is just your estimation? They should be alive but not as happy otherwise.