DNA:RNA hybrid - is it more stable than DNA:DNA or RNA:RNA (Jun/28/2006 )

Hi, all,

I am not very knowledgable at this. How stable is a RNA:DNA duplex in comparison to corresponding DNA or RNA duplex? Is there a site that I can get a formular to predict Tm for DNA:RNA Tm? Thanks in advance.

Here is one might help your question

http://www.sigmaaldrich.com/Brands/Sigma_G...lting_Temp.html

you can also find similar information by google searching

another link
http://www.roche-applied-science.com/PROD_...AN/dig33-37.pdf

generally, a rna.rna hybrid is much more stable than a RNA.DNA which is in turn more stable than a DNA.DNA hybrid. This is basically related to the base stacking and the secondary strucuture and all that. So a RNA.RNA hybrid has a higher Tm than RNA.DNA which in turn has a higher tm than a DNA.DNA duplex.


For calculating tm check this out:
http://www.appliedbiosystems.com/support/techtools/calc/