short temper of my prof....as the last drop! - when world seems to fall apart..... (Jun/22/2006 )
i am really not a depressed type of person who searches for poeple to nag....but these days are just awful... 
i doing 1000 experiments/day to get this protein somehow for the conference that is two weeks away... 
....so im working like crazy, even not seeing my poor baby who decided to spend nights playing so that i cant sleep also... 
...and today my professor comes and says something in really rude mode to me about the incubator which is shaking all night in the refrigirator...he suggested i do it at the first place i need to get temperature to 18 degree to maltiply E-coli....anyway long story short he wouldnt listen to what i was saying just kept talking to me in rude mean voice....and told me to rush up at the end ...as if E-coli can multiply in one hour instead of overnight.... ...i know he has short temper and maybe bad day....and he is really really great researcher and brain....saying all that i just dont know where to find strenght again to make it through these weeks... 
.....his tone is just the last drop! sorry for nagging....
hi
dont worry u will get thru it fine ,, just keep working !!
i have been a similar one last week
had a poster presentation and whatever way i tried i wont get result in my western
all the damn antibodies were wrong !!!!!!!!
had terrible days till i finished it ,, the last moment possible ,,,
and on top it all another guy doing similar expt but different gene got it all
nice and smooth
my protein is very unstable ,, been struggling with it for almost two years now
just try to finish the expt to the last possible step then if u cant complete it , u can say ur still working on that and mention it in future work or something !!!
dont worry about the big boss,, he will calm down when u finally get it done ,, so dont care much about that
they tend to be like that and there is nothing much we can do about it
hang in there , this too will pass !!! and nothing lasts for ever ( thats what i tell myself when the going gets tough)
have faith ,, everything will be alright soon 
all the best
regards
laxmi
The pressure in on for that key piece of data that the abstract hangs on. Keep at it. He must see that you are trying to get the result. He is just annoyed that it will make him look bad if he doesn't have it and therefore he is passing the buck onto you. Not fair, I know but often life isn't fair.
I guess bottom line is that he shouldn't have included that data in the abstract without having it first. (This statement I am assuming that is what he did.)
Your boss looks quite stressed.
His mood will be better after the congress.
keep working hard, the sun will soon shine again.
I guess bottom line is that he shouldn't have included that data in the abstract without having it first. (This statement I am assuming that is what he did.)
Well this happen to my lab as well. Apparently my boss included some data that she "thinks" she can achieve in four months' time into the abstract of a presentation, but she just sat in the office doing nothing all the while till one week ago, she came into the lab, pushed all the work to my labmates, expecting them to produce results in a few days' time. Some bosses are good, some (esp this one that I have) are evil *SIGH*
Anyone have a revenge plan for me?
Kathy, your situation remind me of a story one of my bosses once told me. His boss was giving him a lot of pressure to get some results but there were time limiting steps with the bacteria. At one point, he said to his boss, "What do you want me to do? Whip the cells into growing faster?" His boss then realized he was being unrealistic. Can you imagine someone cracking a whip over a shaking incubator?
Some bosses like to include as much late-breaking data as possible. If you can get the new preliminary data before the conference, it's all gravy. Just do the best you can, presumably you still have a story with the data you already have. Is the results you need for a poster or talk?
The last couple of years, my birthday has been the day before a grant deadline. It is no fun leading up to it and on that day.
These things r common in research. PIs wants to get results as fast as PCR amplification. I guess ur prof. was in a bad mood and somehow u ended up as the victim. Thats Unfortunate !!! Try not to think abt this. Try to focus on what u r doing and keep the momentum. If u start getting worried and start to think abt it ur experiments will suffer. Just pretend as if nothing happeend and think of how happy u will be when u will have ur protein. It will b fine.
Have a good weekend.
thank you everyone for your support....on the top of all that my experiment with purification failed... ....but i took vacation on sunday and feel a lot better today... 
yes i do need those results for the poster presentation but its ok....i will not kill myself for that..... .....
thank you everyone again!