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glycerol - sterilisation (Aug/07/2002 )

Can you sterilise glycerol by autoclaving? If not why?

-sam-

you can do it, but eg. for bacterial freezing stocks it is not necessary.

-snowman-

Glycerol solutions intended for preparation of electro-competent bacteria should be filtered rather than autoclaved for higher transformation efficiency. Glycerol will start to decompose to ethylene aldehyde at its boiling point of 182°C.

-tfitzwater-