euk orthologous database help - need exon-intron boundaries (May/17/2006 )
Recently started an internship in a bioinformatics lab, I've been asked to grab a some orthologous genes for analysis across multiple organisms (H. sapiens, D. melanogaster, G. gallus, C. elegans, A. thaliana and M. musculus). I've looked around GO, TIGR, tried INPARANOID, used homologene on NCBI and a few other things, but can't seem to get what I need.
The number and boundaries of the exons are required, not simply the gene sequence. Maybe I'm missing something, but I can't seem to get this. Homologene will give me lots of sequence, but nothing on exons. If someone knows where to look or what I'm doing wrong, I'd appreciate a shove in the right direction.
Thanks
Do you have the genomic sequences of the organism that you are looking for?
GenScan is a web interface software, that is designed to predict the structure of a given genomic sequences.
http://genes.mit.edu/GENSCAN.html
if you want to search for a similar sequences, try doing BLAST, at NCBI.
Sweetie
Avoid prediction if you can.
Try www.ensembl.org - THIS IS THE DATABASE FOR HIGH QUALITY DATA - EVEN BETTER GO FOR VEGA.
Try www.ensembl.org - THIS IS THE DATABASE FOR HIGH QUALITY DATA - EVEN BETTER GO FOR VEGA.
Also NCBI, have human genome mapped in their site.
Thanks Sweetie and perlmunky.