Rna semi dry transfert overnight... - is it possible? (May/11/2006 )

Hi all.
Well i was wondering if RNA transfert from an acrylamide gel was possible overnight?...
I know that for proteins it's ok but for that purpose?... have you ever done that?

Thanks

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i've never done an rna electrotransfer but i wouldn't use a semi-dry overnight. the filter papers will dry up. semi-dry shouldn't be done for anything significantly greater than 1 hour (maybe 2). the semi-dry apparatus that i use (original abn, later millipore) says not to use for more than 1 hour. some of the other brands may recommend a little longer but not overnight.

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thanks you mfdenko.
For information : my boss do protein electrotransfert overnight at 10v with beautiful results.

Thanks again. That was what i though too but in the other case it would have been interesting, and my manual doesn't tell anything about that

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are his blotting papers still wet when he opens it up? maybe they dry out but after the transfer is complete.

we bought the semi-dry so that we would be able to do quick transfers. we never even thought of transfering overnight and, as i said before, the instructions said not to go over 1 hour. maybe it's because of the type of electrode abn used.

go figure.

thanks for the info.

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