software for gene designing - (May/08/2006 )

Hi,

I would like to design genes for expression in prokaryote aswell as eukaryote.

Can anybody suggest me the best software for synthetic gene design for both prokarytes and eukaryotes.

I couldnt get the one software which can optimise the gene for better expression ( coodn usage,coodn adoptation, mRNA secondary structure , restriction sites, CpG islands, intrinsic transcription terminators, ribosomal binding sites).


I am ready to go for commercial ones.so can anybody plz suggest