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RNA labeled at the 3'-end - (Apr/29/2006 )

hi,everyone, I have a very urgent question:

who knows the protocols about label RNA at the 3'end with isotope?

Thanks in advance!

-sunshine79-

QUOTE (sunshine79 @ Apr 29 2006, 08:36 AM)
hi,everyone, I have a very urgent question:

who knows the protocols about label RNA at the 3'end with isotope?

Thanks in advance!


I am pretty sure that you can use terminal transferase with alpha lablled dNTPs or use RNA ligase with NTPs - have a look in Sambrook or current protocols.

-RWhity-