RNA labeled at the 3'-end - (Apr/29/2006 )
hi,everyone, I have a very urgent question:
who knows the protocols about label RNA at the 3'end with isotope?
Thanks in advance!
-sunshine79-
QUOTE (sunshine79 @ Apr 29 2006, 08:36 AM)
hi,everyone, I have a very urgent question:
who knows the protocols about label RNA at the 3'end with isotope?
Thanks in advance!
who knows the protocols about label RNA at the 3'end with isotope?
Thanks in advance!
I am pretty sure that you can use terminal transferase with alpha lablled dNTPs or use RNA ligase with NTPs - have a look in Sambrook or current protocols.
-RWhity-