Quick help on determining protein size - (Apr/24/2006 )
Hi! If a gene for a protein has 1460 bp, what's the size of the protein produced, how do you determine that?
-smoochiepie79-
you multiplicate by 110, which is the mean weight of aminoacids.
your protein should be around 160 kDa.
However keep in mind that there can be some post translational modifications.
-Missele-
QUOTE (smoochiepie79 @ Apr 25 2006, 12:53 AM)
Hi! If a gene for a protein has 1460 bp, what's the size of the protein produced, how do you determine that?
Hi.
Divide the number of bases by 3 (gives you the number of exons;=486.667). THEN multiply by 110 (average MW of an amino acid residue; =58500 Da; 58 kDa).
-swanny-
Oh yes Swanny you're right, I mixed the bp and the aminoacids !
Then the protein should be around 58 kDa
-Missele-