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how do initiation alpinia galanga (plant culture)? - plant biotechnology (Apr/03/2006 )

hai my name is gerry, i'm a college student in indonesia, i have take the topic initiation of alpinia galanga, i have some trouble, my culture was infected by fungal, and my explan was not grow sad.gif , i don't where the mistakes come from, i sterilized the explant(*buds from rhizome) with NaClO 2,5 (10-15 minutes) % was it not enough?, is there any procedure that i missed ,or my explant is not correct, what is the suitable media for my culture( i have used ms with BA and kinetin),plez help me i have done that experiment since september last year, tengkyu biggrin.gif tongue.gif( i'm sorry if my english bad)

-gerry-

QUOTE (gerry @ Apr 3 2006, 01:49 PM)
hai my name is gerry, i'm a college student in indonesia, i have take the topic initiation of alpinia galanga, i have some trouble, my culture was infected by fungal, and my explan was not grow sad.gif , i don't where the mistakes come from, i sterilized the explant(*buds from rhizome) with NaClO 2,5 (10-15 minutes) % was it not enough?, is there any procedure that i missed ,or my explant is not correct, what is the suitable media for my culture( i have used ms with BA and kinetin),plez help me i have done that experiment since september last year, tengkyu biggrin.gif tongue.gif( i'm sorry if my english bad)


what explant did u took for initiation.have you ever tried mercuric chloride or 70% alcohol for sterilisation. for initiation you can try BAP instead of kinetin

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