Protocol Online logo
Top : Forum Archives: : Immunology and Histology

percol gradient - (Jan/17/2001 )

What cells separate out at the 60,65 and 75% layers when running spleenocytes over a percol gradient? Thanks

--

According to my notes:50%-Macrophages,activated large B cells60%-Small B cells(partially activated)63%-Small B cells(partially activated)66%-Small, resting B cells (1.081-1.086 gm/ml)70%-Pellet: small B cells, RBC's

--