pcDNA4 induction - (Mar/20/2006 )
hi,
can invitrogen pcDNA4.0/Hismax + my insert be induced using IPTG in baterial (BL21-codonplus) ?
thanks
-cyk-
QUOTE (cyk @ Mar 20 2006, 09:21 AM)
hi,
can invitrogen pcDNA4.0/Hismax + my insert be induced using IPTG in baterial (BL21-codonplus) ?
thanks
can invitrogen pcDNA4.0/Hismax + my insert be induced using IPTG in baterial (BL21-codonplus) ?
thanks
Hi,
I am not sure here but I doubt so. This is because pCDNA is a mammalian expression vector and I dont think it contains any IPTG inducible region.
Regards,
tltan
-tltan-