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pcDNA4 induction - (Mar/20/2006 )

hi,

can invitrogen pcDNA4.0/Hismax + my insert be induced using IPTG in baterial (BL21-codonplus) ?

thanks

-cyk-

QUOTE (cyk @ Mar 20 2006, 09:21 AM)
hi,

can invitrogen pcDNA4.0/Hismax + my insert be induced using IPTG in baterial (BL21-codonplus) ?

thanks


Hi,

I am not sure here but I doubt so. This is because pCDNA is a mammalian expression vector and I dont think it contains any IPTG inducible region.

Regards,
tltan

-tltan-