RNAi in yeast? - (Mar/16/2006 )

Hi, I am just starting to work with yeast cells. Would like to try RNAi in yeast. Is it possible at all?, unfortunatelly I found NO protocol available on web. I have good experience with custome made SiRNA from Dharmacon but all in human adherant cells. Thanks A LOT in advance for any tipp!!!!

hi
this article shows an homolog of dicer in yeast.
http://www.pnas.org/cgi/content/abstract/99/26/16648
so i assume rna interference ay be possble as well in yeast...

also this article is kind of a review : Loss of Dicer fowls up centromeres - Nature Cell Biology
www.nature.com/ncb/journal/v6/n8/full/ncb0804-696.html

I appriciate your answer and help a lot. You are a great moderator! THanks
Zlobochka

S.cerevisiae does not have RNAi. Other yeast like S.pombe do have